Name: Duygu Demiröz
E-mail: duygu [dot] demiroz [at] boun [dot] edu [dot] tr
E-mail: dygdmrz [at] yahoo [dot]com
Date of Birth: 31.05.1986
Nationality: Turkish

EDUCATION
Since 2004: Bogazici UNIVERSITY Molecular Biology and Genetics major, Senior Istanbul, Turkey
1997 – 2004: Izmir Anatolian High School – Izmir, Turkey Degree: Excellent

LABORATORY EXPERIENCE
Nov. 2008 – 2013: Assoc. Prof. Nesrin Özören, Bogazici University, Apoptosis and Cancer Research Laboratory
Sep. – Nov. 2008: Assoc. Prof Gamze Köse Yeditepe University, Tissue Engineering Laboratory
RT-PCR and PCR optimization to investigate the presence of certain chondrocyte tissue specific proteins
June – Aug. 2008: Summer Internship Assoc. Prof Gamze Köse Yeditepe University, Tissue Engineering Laboratory
Stem cells and their application for treatment of certain problems in the body. (their application to polymers and examining those with Scanning Electron Microscopy, and also had detailed idea about working principles of Flow Cytometer, Hemocytometer, Confocal Microscope, Fluorescent Microscope, Atomic Force Microscope and Quantitative Real Time PCR
June – Aug. 2007: Prof. Dr. A. Nazli Basak, Bogazici University, Neurodegeneration Research Laboratory
Diagnosis of SCA 1, 2, 3, 6, 7, 17, FRDA and HD
Feb. 2007 – June 2008: Prof. Dr. A. Nazli Basak Neurodegeneration Research Laboratory
Investigation of protein-protein interactions in ALS by two-hybrid screening.
April 2007: Project Manager of ‘Producing Alternative Solutions to Acute Injuries’ whose project team is composed of 4 undergraduates. This project of the Project and Research Club aims to produce a wound covering material that is able to stop haemorrhage in a very short time. For this purpose we are synthesizing a hydrogel polymer and adding a polyphenol with coagulant and antimicrobial activity to it. We will then check for the release pattern of this chemical substance by the hydrogel which we will synthesize in the laboratories of Chemistry Department of Bogazici University.

LABORATORY SKILLS
Culturing human cells
Cryopreservation of cells
Cell culturing methods
Cell counting
Yeast two hybrid system
Bacterial and yeast sterile techniques
Cloning
Transformation, competent cell preparation
Plasmid isolation from yeast and bacterial cells
Agarose gel electrophoresis, DNA isolation from a gel
Spectrophotometer
SDS-PAGE, Western Blotting
NaCl DNA extraction from blood samples
Diagnosis of certain neurological disorders (SCA 1, 2, 3, 6, 7, 17, FRDA and HD)
Western blotting
Standard PCR, Quantitative Real Time PCR
Pure cell culture techniques
Simple and differential staining methods
Basic organic laboratory techniques (recrystallization, liquid-liquid and solid-liquid extractions, simple and fractional distillation, sublimation)
Column and paper chromatography
Culturing of D.melanogaster and their monohybrid and dihybrid crosses
Blood smear preparation and slide examination
Measuring blood pressure
Reading Electrocardiograms
Light microscopy

CONFERENCES AND ORGANIZATIONS
May 09 – 10, 2008: Organization of Project and Research Conference as a Project and Research Club
April 09, 2008: Attendance of Programmed Cell Death Symposium Istanbul University Cerrahpasa Medical Faculty, Istanbul, Turkey
October 5 – 6, 2007: Organization committee member in the COST conference ‘Cost B30: Neural Regeneration And Plasticity’ organized by Prof. Dr. A. Nazli Basak in Istanbul
April 27, 2007: Attendance to ‘Risk Management’ as a continuation of the seminar series 2007 of Istanbul Technical University -instructed by Project Management Professional Arpat Ömür, Certified by Project Management Institute.
April 5 – 8, 2007: Organization committee member in the conference ‘New Frontiers in Understanding and Treating ALS’ coordinated by Prof. Dr. A. Nazli Basak and Prof. Robert Brown in Istanbul
March 24 – 25, 2007: Attendance to ‘Project Management Basis’ to the seminar series 2007 of Istanbul Technical University instructed by Project Management Professional Ahmet Taspinar and Assoc. Prof. Attila Dikbas Certified by the Project Management Institute
Dec 2006 – Jan 2007: Organization of the exhibition ‘James D. Watson: Journey to the DNA world’ with Prof. Dr. A. Nazli Basak
December 18, 2006: Participation to the Structural Biology Training Program (including structural determination program PyMOL)

SOCIAL ACTIVITIES
June, 2007- Membership of Bogazici University Project and Research Club’s Auditing Committee
Oct, 2006 – June, 2007 Secretary of Bogazici University Project and Research Club’s Project Sub-committee
Oct, 2004 – June, 2005 Bogazici University Social Service SocietyWorking as a volunteer mathematics teacher in OSS (Nationwide University Entrance Exam) Training Project that was developed together with TOG for one year.
Oct, 2004 – June, 2005 Bogazici University Engineering Society Organization committee member of the Career Solutions Program

LANGUAGE SKILLS
Turkish: Native
English: Fluent
German: Elementary
Spanish: Intermediate

COMPUTER SKILLS
MS Office (XP and 2007) usage (MS Word, MS Excel, MS PowerPoint, MS Project)
Windows XP user

HOBBIES
Making pieces of jewels and knitting
Painting wooden material
Ceramics
Cycling